Table 1.
Genetic Interactions between abi8 and Other ABA Response Loci
| Genotype | Germination (%) 0.3 μM ABA | (n) | Germination (%) 1 μM ABA | (n) | Dwarf (%) | (n) |
|---|---|---|---|---|---|---|
| Ws | 73.3 | (359) | 19.1 | (393) | 0 | |
| abi8/+ | 96.4 | (501) | 49.2 | (528) | 22 | (463) |
| abi8 | 100 | (125) | 100 | (132) | ||
| 35S:ABI3 | 45.2 | (126) | 16.9 | (136) | 0 | |
| abi8/+ 35S:ABI3 | 68 | (409) | 21.4 | (252) | 20.4 | (216) |
| abi8 35S:ABI3 | 92.7 | (82) | 80.7 | (88) | ||
| 35S:ABI5 | 2 | (197) | 0 | (181) | 0 | |
| abi8/+ 35S:ABI5 | 26.7 | (533) | 15.6 | (449) | 24.1 | (429) |
| abi8 35S:ABI5 | 54.1 | (133) | 62.5 | (112) | ||
| abh1 | 31.4 | (118) | 1.5 | (134) | 0 | |
| abi8/+ abh1 | 15.4 | (280) | 4.3 | (232) | 23.2 | (302) |
| abi8 abh1 | 57 | (70) | 17.3 | (58) |
ABA sensitivity was assayed by measuring germination (scored as emergence of entire seedling from seed coat) after 4 d of incubation on 0.3 μM ABA or 8 d of incubation on 1 μM ABA. Among germinating progeny of abi8/+ 35S:ABI or abi8/+ plants, abi8 segregants were identified by their swollen radicles. The normalized percentage of germination was calculated relative to the anticipated fraction of double homozygotes. Dwarfing as a result of the abi8 mutation was assayed in progeny of abi8/+ plants grown on hormone-free medium; dwarfing was not observed in lines lacking the abi8 mutation. The results are expressed as a percentage of the total number of seeds plated (n).