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. Author manuscript; available in PMC: 2012 Aug 14.
Published in final edited form as: Eur J Immunol. 2009 Dec;39(12):3395–3403. doi: 10.1002/eji.200939587

Fig. 6.

Fig. 6

Reduced IgG1 production by gp91phox KO B cells in vitro does not correlate with dysregulated in vivo T-dependent antibody production by gp91phox KO mice. (A) WT (diamonds) or gp91phox KO (circles) B cells were cultured with medium alone (0) or 0.3, 1.0 or 3.0 μg/mL anti-CD40 plus 10 ng/mL IL-4 plus. After seven days, supernatants were collected and analyzed by ELISA. ***p≤0.0007, **p≤0.008, *p≤ 0.02; Student’s t-test, three mice per group. (B) WT (diamonds) and gp91phox KO (circles) mice were injected intra-peritoneally with either 5 μg (open) or 50 μg (closed) alum-precipitated DNP-KLH. Sera were collected prior to immunization and then every week for three weeks. Ag-specific antibody titers were measured by ELISA. n.s., not significant, student’s t-test. Data show mean + SD, n= 5 mice per group.