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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: Cancer Res. 2011 Oct 10;71(24):7670–7682. doi: 10.1158/0008-5472.CAN-11-0964

Figure 4. ETS-1 regulation by miR-200.

Figure 4

A. Quantitative RT-PCR analysis of ETS-1 mRNA levels in 344SQ or 393P cells stably transfected with an empty vector control (WT), the miR-200b/a/429 locus (200b), or Zeb1 normalized on the basis of L32 ribosomal protein mRNA levels and expressed as mean values of triplicate cultures relative to control transfectants, which were set at 1.0. B. Western blot analysis of the same cell lines. C. 344SQ cells were transiently co-transfected with the indicated pre-miRs or scrambled oligomer (10 nM) and reporter plasmids (500 ng) that are linked to the full-length 3′-UTR of ETS-1 (left) or Zeb1 (right). Results were normalized on the basis of renilla luciferase and expressed as the mean values of triplicate wells. * p<0.05. D. Map of 3′UTR’s for luciferase assays showing miR-200 target sites.