Table 1.
Summary of the in Vitro and in Vivo Activities of the Wild-Type and Activated Forms of SOS2
|
S. cerevisiae Growth (in Salt)
|
A. thaliana Growth (in Salt)
|
||||||
|---|---|---|---|---|---|---|---|
| Autophosphorylation | Peptide Phosphorylation | +SOS3 | −SOS3 | sos2 | sos3 | WT | |
| SOS2 | − | − | ++++ | − | + | − | − |
| T/DSOS2 | ++ | ++ | ++++ | − | + | + | + |
| T/DSOS2DF | ++ | +++ | + | + | + | − | + |
| T/DSOS2/329 | + | + | ++ | + | − | − | ND |
| T/DSOS2/308 | +++ | ++++ | ++ | ++ | − | − | ND |
The level of phosphorylation activity in vitro and relative salt tolerance in vivo are indicated for each SOS2 variant by the number of + symbols. Minus symbol indicates undetectable phosphorylation or no growth. ND, not determined; WT, wild-type form of SOS2.