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. 2012 Aug 14;7(8):e43072. doi: 10.1371/journal.pone.0043072

Figure 3. Time course recordings of purified pHlash protein at three different pHs in vitro.

Figure 3

(A) pH 6.9, (B) pH 7.5, and (C) pH 8.1. (D) While the total luminescence intensity tends to decline over time after the addition of substrate, the BRET ratio (525∶475 nm) is constant over time. (E) Comparison of BRET ratio calibration measured in vitro by two different methods, the “Emission scan mode” and the “Two-Wavelength Switching Mode” (see Methods for details). Error bars are +/− S.D., but in most cases the error bars are so small that they are obscured by the symbols (n = 3).