Table 1. T_H17 cell antigens isolated from immunogenic fractions of soluble portion of RM200, predicted function, and primers used for expression in E. coli.

Locus in TIGR4	Predicted function	Primer sequence (5′–3′) using Bam HI and SphI restriction sites
SP 0435	Elongation factor	up- GCGGATCCATGATTGAAGCAAGTAAAT down- GCGCATGCTTAGGCACGAGAAACGTAAG
SP 0516	Heat shock protein	up- GCGGATCCATGGCCCAAGATATAAAAAATGAAG down- GCGCATGCTTAGTTATACACCACTACCATT
SP 0862	Ribosomal protein S1	up- GCGGATCCATGAACGAATTTGAAGATTTGC down- GCGCATGCTTAAAGTTCGATATCACCAAACAAATC
SP 0946	Hypothetical	up- GCGGATCCATGAATACAAATCTTGCAAG down- GCGCATGCCTAAATCAACTCTGTCCC
SP 1297	Flavodoxin	up- GCGGATCCATGGCATTAGCAAAAATTGTATTTG down- GCGCATGCTTATCCTACTTTAGCAGCCAATTC
SP 1415	Glucosamine-6-phosphpate isomerase	up- GCGGATCCATGAAAGTTATTAAAGTTGAAAAC down- GCGCATGCTTAGAGTAAGCTAAGCGCTTCAGC
SP 1458	Thioredoxin reductase	up- GCGGATCCATGTACGATACTATTATTATCGG down- GCGCATGCTTAACTATGTTCTGTAATGAATT
SP 1534	Putative manganese-dependent inorganic pyrophosphatase	up- GCGGATCCATGTCCAAGATTCTAGTATTTG down- GCGCATGCTTACGCATTAAAGCTTTCAGTC
SP 1572	Non-heme iron containing ferritin	up- GCGGATCCATGAATGAGGTAAAGAAAATGG down- GCGCATGCTTACAAACCAGGTGCTTGTCCAAG
SP 1733	Putative phosphatase	up- GCGGATCCATGGAAATTTCATTATTAACAGATG down- GCGCATGCTCATTCTGCATCCTCCTCGTTC
SP 2070	Glucose-6-phosphate isomerase	up- GCGGATCC ATGTCACATATTAAATTTGATTATTC down- GCGCATGCTTATAGACGTGCGTTAAGTTCTTTG
SP 2092	UTP-glucose-1-phosphate uridyltransferase	up- GCGGATCC ATGACATCAAAAGTTAGAAAGGC down- GCGCATGCTTATTCCTTCTCAGTCAATTCTTTTC