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. 2012 Aug 14;7(8):e42514. doi: 10.1371/journal.pone.0042514

Figure 3. Minimum Spanning Tree analysis of published B. melitensis isolates (excluding vaccine strains) using the MLVA11 data.

Figure 3

Colour codes are associated with the main B. melitensis MLVA groups. The analysis was performed using all isolates from this study and typing data from more than one thousand isolates previously published [19]–[22], Brucella2010 database hosted at http://mlva.u-psud.fr]. Portuguese isolates are shown in yellow. The B. melitensis population is included in “American” (17%) and “East-Mediterranean” (83%) groups. Thirty nine human isolates (85%; 39 out of 46) are MLVA11 genotype 116 usually associated with the “East-Mediterranean” B. melitensis strains and seven isolates (15%) are included in the “American” group. The numbers represent the MLVA11 genotypes found in this study. The size of the shapes indicates the number of strains described in each genotype. Each of the circles showing yellow and dark blue or red colors included the Portuguese genotype (yellow) and the genotypes found in “East-Mediterranean” (dark blue) or “American” (red) B. melitensis isolates.