Figure 6. ITR sequence analyses.

Sequences of the Mos1-tribe ITRs (Tables 1 and 2) were aligned to obtain 5′ and 3′ITRs logos and consensus. The majority rule was used to define variable positions, which are 1, 2, 3, 14, 18, and 26 in the 5′ITR and 1, 2, 3, and 14 in the 3′ITR. Doing so, the 24 conserved positions in Mos1 ITRs are conserved in the consensus ITRs (black letters). When compared to each other, 5′ and 3′ consensus ITRs contain three clear differences at positions 1, 18, and 26, which are the same as that found in Mos1 ITRs (blue letters). Position 16 (purple letter) in the 5′ consensus remains ambiguous, being either a G (as in the 5′ Mos1 ITR) or a T (as in the 3′ Mos1 ITR).