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. 2012 Aug 14;7(8):e43187. doi: 10.1371/journal.pone.0043187

Figure 5. IP6 directly stimulates gene expression of osteoclast functional markers and resorption activity on mature osteoclasts-like cells.

Figure 5

Mature osteoclasts were treated with 1 µM of IP6 for 24 hours and gene expression of osteoclast functional markers was determined: Car-2(A), H+-ATPase (B), CtsK (C) and Mmp-9 (D). Data represent fold changes of target genes normalized with Gapdh mRNA and 18s rRNA, expressed as a percentage of RANKL-dosed cells non-treated with IP6, which were set to 100%. Values represent the mean ± SEM. Significant differences were assessed by Student’s t test: *p≤0.05 versus control cells. (E) Bone resorption ability of mature osteoclasts treated with 1 µM of IP6 for four days was evaluated by resorption pit assay on dentine discs. Data represent the percentage of the resorbed area by mature osteoclasts. Values represent the mean ± SEM. Significant differences were assessed by Student’s t test: *p≤0.05 versus untreated cells. #p≤0.05 versus RANKL treated cells. (n = 3).