Figure 1.

Identification of DMRs in Endosperm DNA by MSAP.

Representative MSAP profiles of an EcoRI/HpaII digest of endosperm DNA extracted from the inbred lines W64A (W) and A69Y (Y) and the reciprocal crosses W64/A69Y (W/Y) and A69Y/W64 (Y/W) (the seed parent of the reciprocal cross is underlined) harvested at 15 DAP. The sum of profiles scored of both inbred lines using 24 selective primer combinations is indicated at the right of each panel.

(A) An MSAP restriction fragment specific to the A69Y inbred line that did not show parental differences in methylation (a normal polymorphic profile).

(B) MSAP restriction fragments that only were detected when the A69Y inbred line was the seed parent (maternal profiles are indicated by arrows).

(C) MSAP restriction fragments that only were detected when the W64A inbred line was the pollen parent (paternal profiles are indicated by arrows).

(D) Monomorphic profiles.