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. 2012 Aug 14;7(8):e43255. doi: 10.1371/journal.pone.0043255

Figure 7. Quantitative cytokine analysis and acquisition of metabolic parameters of single-step and two-step MSC expansion media.

Figure 7

Samples of cell culture supernatants were collected at the time points indicated during single-step and two-step (TSP1; 14+7-5-8) GMP-grade large-scale cell expansion runs and analyzed for their cytokine content. Depicted are results of glucose, lactate, bFGF, IL-6, GRO (CXCL1/2/3), sCD40L, RANTES/CCL5, PDGF-AA, VEGF (n = 4 single step runs; n = 3 two-step runs), sVCAM-1, PDGF-AB/BB (n = 3 single step runs; n = 3 two-step runs), sICAM-1, and TGF-β1 (n = 2 single step runs; n = 3 two-step runs) contents as mean and SD values.