Table 2. Comparison of the different protocols and their outcomes.

System	single-step	two-step
Protocol	SSP	TSP1	TSP2	TSP3	TSP4
Units needed to obtain100×106 MSC*	3×5-chamberstack	p0: 1×2-chamber stack p1: 2×2-chamber stack	p0: 1×2-chamber stack p1: 3×2-chamber stack	p0: 1×2-chamber stack p1: 3×2-chamber stack	p0: 1×2-chamber stack p1: 2×2-chamber stack
Volume of BM needed toseed this number of stacksin p0	12.7 mL	1.9 mL	2.2 mL	2.2 mL	2.0 mL
Volume of mediumneeded to seed thisnumber of stacks	6.0 L	2.1 L	1.5 L	2.1 L	1.5 L
Volume of PL needed toseed this number of stacks	600 mL	132 mL	93 mL	210 mL	150 mL
Overall expansion time	12.0 days	20.7 days	14.7 days	20.8 days	14.8 days
Population doublingsper day	1.08	1.05	1.01	0.76	0.77
Trilineage-differentiationcapacity	passed	passed	passed	passed	passed
Surface marker expressionof standard panel	passed	passed	passed	passed	passed
Surface marker expressionof extended panel;at p0 to p>1	increasing expressionof integrins α4 and αv, loss of CD46 and MSCA-1	loss of expression of integrins α4 and αv, stable expression of CD146 and MSCA-1

^*always rounded up to next higher integer. All values represent mean values.