Figure 5. Silencing of ATG5 gene.

MDA-MB-231 cells were transfected with siRNA targeting ATG5 gene (siATG5) and control siRNA as described in methods. (A) Time-dependent decrease in ATG5 protein in siATG5-transfected cells. (B) CTet activity in ATG5-depleted cells. The ATG5 gene silencing greatly increases cell sensitivity to CTet compared to control siRNA- or non-transfected cells. Data are means ± SEM of three experiments.