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. 2012 Aug 14;7(8):e43249. doi: 10.1371/journal.pone.0043249

Table 2. Primer sequences for SYBR green RT-qPCR.

Target mRNA Accession number Forward primer (5′–3′) Reverse primer (5′–3′) Amplicon lenght (bp) Ref.
MAP1LC3B NM_022818 AAACGGGCTGTGTGAGAAAAC TGAGGACTTTGGGTGTGGTTC 83
DDIT3 NM_001195053 GGAGCATCAGTCCCCCACTT TGTGGGATTGAGGGTCACATC 101 [35]
CHAC1 NM_024111 TGGTGACGCTCCTTGAAGATC GCACTGCCTCTCGCACATT 111
ATF3 NM_001674 GCCATCCAGAACAAGCACCT GGCTACCTCGGCTTTTGTGAT 105
HSPA5 NM_005347 CCCCGAGAACACGGTCTTT CAACCACCTTGAACGGCAA 101
CEBPB NM_005194 CGAAGTTGATGCAATCGGTTT TTAAGCGATTACTCAGGGCCC 101
ASNS NM_133436 ATTGGCTGCCTTTTATCAGGG TGTCTTCCATGCCAATTGCA 121
GAPDH NM_002046 CCATGTTCGTCATGGGTGTG GGTGCTAAGCAGTTGGTGGTG 91

MAP1LC3B (microtubule-associated protein 1 light chain 3 beta); DDIT3 (DNA-damage-inducible transcript 3); CHAC1 [ChaC, cation transport regulator homolog 1 (E. coli)]; ATF3 (activating transcription factor 3); HSPA5 [heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa)]; CEBPB [CCAAT/enhancer binding protein (C/EBP), beta]; ASNS (asparagine synthetase); GAPDH (glyceraldehyde-3-phosphate dehydrogenase).