Table 2. Primer sequences for SYBR green RT-qPCR.
Target mRNA | Accession number | Forward primer (5′–3′) | Reverse primer (5′–3′) | Amplicon lenght (bp) | Ref. |
MAP1LC3B | NM_022818 | AAACGGGCTGTGTGAGAAAAC | TGAGGACTTTGGGTGTGGTTC | 83 | |
DDIT3 | NM_001195053 | GGAGCATCAGTCCCCCACTT | TGTGGGATTGAGGGTCACATC | 101 | [35] |
CHAC1 | NM_024111 | TGGTGACGCTCCTTGAAGATC | GCACTGCCTCTCGCACATT | 111 | |
ATF3 | NM_001674 | GCCATCCAGAACAAGCACCT | GGCTACCTCGGCTTTTGTGAT | 105 | |
HSPA5 | NM_005347 | CCCCGAGAACACGGTCTTT | CAACCACCTTGAACGGCAA | 101 | |
CEBPB | NM_005194 | CGAAGTTGATGCAATCGGTTT | TTAAGCGATTACTCAGGGCCC | 101 | |
ASNS | NM_133436 | ATTGGCTGCCTTTTATCAGGG | TGTCTTCCATGCCAATTGCA | 121 | |
GAPDH | NM_002046 | CCATGTTCGTCATGGGTGTG | GGTGCTAAGCAGTTGGTGGTG | 91 |
MAP1LC3B (microtubule-associated protein 1 light chain 3 beta); DDIT3 (DNA-damage-inducible transcript 3); CHAC1 [ChaC, cation transport regulator homolog 1 (E. coli)]; ATF3 (activating transcription factor 3); HSPA5 [heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa)]; CEBPB [CCAAT/enhancer binding protein (C/EBP), beta]; ASNS (asparagine synthetase); GAPDH (glyceraldehyde-3-phosphate dehydrogenase).