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. 2012 Aug 14;6(8):e1770. doi: 10.1371/journal.pntd.0001770

Table 2. Effect of tpp gene silencing on viability of both adult male and female parasites of B. malayi was assessed by motility scoring and MTT reduction assay.

Motility score (female worms) % Inhibition in MTT reduction Motility score (male worms) % Inhibition in MTT reduction
Time Control Negative siRNA Gene specific siRNA Negative siRNA Gene specific siRNA Control Negative siRNA Gene specific siRNA Negative siRNA Gene specific siRNA
12 5 5 3 6.03±4.1 48.52±7.8 5 5 3 6.16±5.8 48.16±8.2
24 5 5 3 5.6±1.6 49.9±11.1 5 5 2 6.8±4.3 60.5±8.1
36 4 5 2 6.16±1.6 63.8±13.1 4 4 2 6.6±3.9 64.83±8.5
48 4 4 2 6.66±1.0 73.92±2.6 4 4 2 9.3±5.9 74.5±3.2
108 h 4 4 2 6.9±2.2 78.18±5.1 4 4 1 11.33±9.6 87.33±6.2

5 = 0% motility reduction, 1 to 25% reduction = 4, 26 to 49% reduction = 3, 50 to 74% reduction = 2; 75 to 99% reduction = 1, 100% = Death.