Table 2. Effect of tpp gene silencing on viability of both adult male and female parasites of B. malayi was assessed by motility scoring and MTT reduction assay.
Motility score (female worms) | % Inhibition in MTT reduction | Motility score (male worms) | % Inhibition in MTT reduction | |||||||
Time | Control | Negative siRNA | Gene specific siRNA | Negative siRNA | Gene specific siRNA | Control | Negative siRNA | Gene specific siRNA | Negative siRNA | Gene specific siRNA |
12 | 5 | 5 | 3 | 6.03±4.1 | 48.52±7.8 | 5 | 5 | 3 | 6.16±5.8 | 48.16±8.2 |
24 | 5 | 5 | 3 | 5.6±1.6 | 49.9±11.1 | 5 | 5 | 2 | 6.8±4.3 | 60.5±8.1 |
36 | 4 | 5 | 2 | 6.16±1.6 | 63.8±13.1 | 4 | 4 | 2 | 6.6±3.9 | 64.83±8.5 |
48 | 4 | 4 | 2 | 6.66±1.0 | 73.92±2.6 | 4 | 4 | 2 | 9.3±5.9 | 74.5±3.2 |
108 h | 4 | 4 | 2 | 6.9±2.2 | 78.18±5.1 | 4 | 4 | 1 | 11.33±9.6 | 87.33±6.2 |
5 = 0% motility reduction, 1 to 25% reduction = 4, 26 to 49% reduction = 3, 50 to 74% reduction = 2; 75 to 99% reduction = 1, 100% = Death.