Figure 5. TRPA1 mediates IL-8 release by cigarette smoke extract from human airway/lung cells in primary culture.

IL-8 release induced by overnight exposure to increasing concentrations (expressed as optical density, OD) of cigarette smoke extract (CSE) in small airway epithelial cells (SAEC) (A), normal human lung fibroblasts (NHLF) (B) and human bronchial smooth muscle cells (HBSMC) (C). IL-8 release evoked by CSE is reduced by HC-030031 (HC, 30 µM) and AP18 (10 µM). Each column represents mean ± SEM of at least 3 independent experiments. ^§ P<0.05 vs. Basal group or Veh/Veh-CSE; ^* P<0.05 vs. Veh/CSE.