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. 2012 Aug 14;7(8):e42874. doi: 10.1371/journal.pone.0042874

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© 2012 Huynh et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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P. aeruginosa PAO1 WT (grey) and cyaA mutant (black) biofilm formation and response to starvation was determined by biofilm-opacity measurement (A). Percentage of of cyaA biomass remaining after 1 day of glucose starvation (p<0.05) as determined by the biofilm-opacity monitoring system (B); error bars represent standard error. Biofilm formation and response to glucose starvation of P. aeruginosa PAO1 WT (grey) and complemented cyaA mutant (black) as determined by biofilm-opacity measurement (C). Glucose starvation was induced at the time shown by the vertical line.