Figure 7. Effect of the inhibition of cAMP synthesis on the dispersal of PAO1 WT biofilms.

The effect of inhibition of cAMP synthesis on dispersal of biofilms grown under continuous flow conditions in M9 medium (100 µM CaCl₂) or M9 medium containing 7 mM atropine (Ap) as assessed photometrically (A) and percentage of biomass remaining after 1 day of glucose starvation with and without 7 mM Ap (p<0.05) (B) were determined. Intracellular cAMP levels in WT, cyaA mutant and Ap-treated P. aeruginosa planktonic cells were determined (C). Effect of CCCP and arsenate treatment on intracellular levels of cAMP in P. aeruginosa grown in planktonic culture (D). Two sets of samples were prepared on different days, and were measured in triplicate (n = 6). Error bars indicate standard error.