Figure 3.

Positional Cloning of the REF1 Gene.

The REF1 gene was mapped to a 154-kb region between markers Cer435669 and g4711 using a mapping population of 550 F2 plants. This region is covered by three P1 clones containing 39 annotated genes. No recombinants were found with marker Cer438851 located on the P1 clone MOB24, suggesting a very tight linkage of the REF1 gene to this marker. A putative ALDH gene located <8 kb from the Cer43851 marker was identified as the REF1 gene by mutant complementation and sequence analysis.