Figure 1.

Albumin, IgG, and plasmin were present in PVR vitreous. (A) Vitreous from rabbits and patient was tested for albumin, IgG, and plasmin. Left: C, vitreous from control (uninjected) eyes; 1, 3, and 5 indicate the stage of PVR; P, 10% platelet-rich plasma. For the patient vitreous (right), samples 4, 9, 10, 12, 19, 22, 27, and 49 were from PVR patients, and sample 2 was from a non-PVR patient. Samples (1 μL rabbit vitreous or 10 μL patient vitreous) were separated by 10% SDS-PAGE, and proteins in the resultant gel were visualized by Coomassie brilliant blue staining (top row). A parallel set of samples was subjected to Western blot analysis using antibodies against albumin (second row from the top), IgG (third row from the top), or plasmin (bottom row). The data shown are representative of the entire data set, which included vitreous from 8 control rabbit eyes, 22 PVR rabbit eyes (3, 5, and 14 [stages 1, 3, and 5, respectively]), 10 patients with PVR, and 16 non-PVR patients. (B, C) The intensity of Western blot bands for plasmin of all rabbit and patient samples was quantitated, and the resultant data are presented as mean ± SD. Comparison of the plasmin band intensity with known amounts of purified plasmin standards permitted calculation of the amount of plasmin in each of the samples. (B) 0.31 ± 0.05, 1.06 ± 0.15, 2.07 ± 0.26, 11.3 ± 1.89, and 1.55 ± 0.15 nM plasmin for C, 1, 3, 5, and P, respectively. Similar analysis indicated 29.2 ± 9.8 nM plasmin in the vitreous of patients with PVR.