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. Author manuscript; available in PMC: 2012 Aug 14.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Jan;49(1):42–48. doi: 10.1167/iovs.07-0776

Figure 4.

Figure 4

Plasmin processed PDGF-C better than tPA. RPE-conditioned medium (38 μL) containing latent native PDGF-C was incubated with plasmin (25 nM) or tPA (25 nM) for the indicated times at 37°C. This is the optimum concentration for tPA10 and was slightly below the optimum concentration for plasmin (30 mM). The reactions were stopped, and the samples were subjected to PDGF-C Western blot analysis. In three independent experiments, we found that 33.9% ± 3.5% PDGF-C was processed in 5 minutes by plasmin, whereas it took 960 minutes for tPA to process this amount of PDGF-C.