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. Author manuscript; available in PMC: 2012 Aug 14.
Published in final edited form as: Contrast Media Mol Imaging. 2010 May 28;6(1):55–59. doi: 10.1002/cmmi.395

Figure 1.

Figure 1

Emission light spectra of BRET-Qdot655 excited by the incubation with coelenterazine in in vitro (a) and in vivo (b). Two emission peaks from bioluminescence (luciferase) and BRET (Qdot655) are identified in vitro; however, only one peak from BRET signal (Qdot655) is detected in vivo. Note that a mixture of BRET-Qdot655 and coelenterazine was injected subcutaneously for in vivo BRET spectral imaging. Also note that the emission peak from bioluminescence is shifted to longer wavelength because of the 515 nm long-pass emission filter.