Figure 3.
A Complex Interaction Network Is Occurring at the Level of the Nucleotide and PspF Interface
(A) ATPase activity of the mixed cis (N64, D107, and E108) and trans (R162, D164, and R168) defective variants. Histograms represent the kcat observed for the different variants mixed at equimolar concentration. Experiment was performed three times independently, and the maximal error observed was below 10%.
(B and C) Schematic of PspF-ATP (B, pdb 2C9C) and PspF-ADP (C, pdb 2C98) interaction networks. Dashed black lanes represent polar contact between side chains, and purple dashed lanes represent polar contact with the solvent. Note that R122 side chain is not resolved in the ATP soaked crystal. Figure was generated using PyMol software.