Figure 3.

Autophagy Is Increased in Bim-Knockout Mice

(A) Lysates from spleen-derived T cells of wild-type (Bim^+/+) and Bim-knockout (Bim^−/−) mice were immunoblotted and probed with indicated antibodies. Statistics were from three knockout and control mice. Data are shown as mean ± SD. ^∗∗∗p < 0.001.

(B) T cells from Bim^+/+ and Bim^−/− mice were cultured with/without Baf for 4 hr preceding harvest, immunoblotted, and probed with the indicated antibodies.

(C) Spleen-derived T cells from Bim^+/+ or Bim^−/− mice were stained with rabbit anti-LC3 antibody. Representative confocal images and quantification from triplicates (n = 100–110) are shown. Data are shown as mean ± SD. ^∗∗∗p < 0.0001.

(D) Bim^+/+ and Bim^−/− mouse liver lysates were immunoblotted and probed with the indicated antibodies (n = 3). Data are shown as mean ± SD. ^∗∗p < 0.01.

(E) Bim^+/+ and Bim^−/− mouse liver lysates were immunoblotted and probed with the indicated antibodies (n = 3). Data are shown as mean ± SD. ^∗∗∗p < 0.001.

(F) RNA from Bim wild-type or Bim-knockout mouse livers was analyzed by qRT-PCR for p62/beta-actin mRNA. The mean ± SD from three mice per group is shown. NS, not significant.

See also Figure S3.