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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Mol Aspects Med. 2012 Feb 11;33(5-6):553–561. doi: 10.1016/j.mam.2012.02.001

Figure 2.

Figure 2

Reversible ion channel activation of Drosophila Big Brain aquaporins expressed in Xenopus oocytes.

(A) Reversible activation of the Big Brain (BIB) ionic conductance measured by twoelectrode voltage clamp of an oocyte expressing hemagglutinin (HA) epitope-tagged BIB channels.

(B) Confocal imaging with anti-HA antibody labeling of BIB protein, showing expression in Xenopus oocyte plasma membranes for wild type channels and for a non-ion conducting mutant (glutamate 71 to asparagine) which exerts a dominant negative effect on ionic conductance activity.