Skip to main content
. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Dev Growth Differ. 2012 Jul 8;54(6):633–648. doi: 10.1111/j.1440-169X.2012.01364.x

Fig. 2. TGFβ3 regulates E-Cadherin, Fibronectin, Vimentin and N-Cadherin.

Fig. 2

Single staining immunofluorescence (FITC) protein expression assays were performed on homogenous MES cells treated with TGFβ3 (5ng/mL) for 24h and 48 h. E-Cadherin (A, B & C), Desmoplakin (D, E & F), ZO1 (G, H & I) and Keratin 14 (J, K & L) were highly expressed at the cell membrane of untreated (control, A, D, G & J) MES cells, reduced by 24 h (B, E, H and K), and lost by 48 h of TGFβ3 treatment (C, F, I and L). Unlike other three epithelial markers, Keratin 14, however, is expressed in the cell membrane and in the cytoplasm in untreated cells. And is relocated mostly in the cytoplasm in response to TGFβ3 (24h) before completely repressed by 48h