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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Dev Growth Differ. 2012 Jul 8;54(6):633–648. doi: 10.1111/j.1440-169X.2012.01364.x

Fig. 3. TGFβ3 regulates Desmoplakin, Fibronectin and N-Cadherin.

Fig. 3

Double staining immunofluorescence protein expression assays were performed on homogenous MES cells that were treated with TGFβ3 (5ng/mL) for 24 and 48 h. In double staining, E-Cadherin (A, Rhodamine), Desmoplakin (D, Rhodamine) and ZO1 (G, FITC) were highly expressed while Fibronectin (A, FITC), N-Cadherin (D, FITC) and Vimentin (G, Rhodamine) expression were not seen in untreated control cells. As expression of Fibronectin, Vimentin, and N-Cadherin began to increase, in response TGFβ3 for 24 h (B, E & H), E-Cadherin, Desmoplakin and ZO1 expressions were reduced and significantly lost by 48 h (C, F & I), at which stage, Fibronectin, Vimentin, and N-Cadherin were highly expressed.