Figure 4.

Physical analysis of B. pseudomallei CPS-cBSA glycoconjugates. (A) SDS-PAGE and Coomassie Blue staining was used to confirm the covalent linkage of BP2683 CPS to cBSA. The positions of protein molecular size standards are indicated on the left. (B) Western immunobloting was also used to assess the antigenicity of the chemically activated/coupled BP2683 CPS. The CPS + cBSA lane represents unconjugated controls. CPS was detected using the 3C5 mAb. Lanes were loaded with similar amounts of protein or carbohydrate to facilitate direct comparisons.