Table 2.
E. coli-resistant antibiotic genes, primer sequence, and product size.
| Antibiotic | Resistant gene | Sequence | Size (bp) | Annealing temperature (°C) | References |
|---|---|---|---|---|---|
| Streptomycin | aadA1 | (F) TATCCAGCTAAGCGCGAACT (R) ATTTGCCGACTACCTTGGTC |
447 | 58 | [30] |
| Tetracycline | tetA | (F) GGTTCACTCGAACGACGTCA (R) CTGTCCGACAAGTTGCATGA |
577 | 57 | [30] |
| Tetracycline | tetB | (F) CCTCAGCTTCTCAACGCGTG (R) GCACCTTGCTGATGACTCTT |
634 | 56 | [30] |
| Trimethoprim | dfrA1 | (F) GGAGTGCCAAAGGTGAACAGC (R) GAGGCGAAGTCTTGGGTAAAAAC |
367 | 45 | [31] |
| Fluoroquinolone | qnr | (F) GGGTATGGATATTATTGATAAAG (R) CTAATCCGGCAGCACTATTTA |
670 | 50 | [32] |
| Gentamicin | aac(3)-IV | (F) CTTCAGGATGGCAAGTTGGT (R) TCATCTCGTTCTCCGCTCAT |
286 | 55 | [33] |
| Sulfonamide | Sul1 | (F) TTCGGCATTCTGAATCTCAC (R) ATGATCTAACCCTCGGTCTC |
822 | 47 | [33] |
| Cephalothin | blaSHV | (F) TCGCCTGTGTATTATCTCCC (R) CGCAGATAAATCACCACAATG |
768 | 52 | [33] |
| Ampicillin | CITM | (F) TGGCCAGAACTGACAGGCAAA (R) TTTCTCCTGAACGTGGCTGGC |
462 | 47 | [33] |
| Chloramphenicol | cat1 | (F) AGTTGCTCAATGTACCTATAACC (R) TTGTAATTCATTAAGCATTCTGCC |
547 | 55 | [33] |
| Chloramphenicol | cmlA | (F) CCGCCACGGTGTTGTTGTTATC (R) CACCTTGCCTGCCCATCATTAG |
698 | 55 | [33] |