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Normal human SMC and aortic EC (passage 2) cultured separately and together (SMC + EC). (a) Immunofluorescence expression of Von Willebrand factor (previously known as factor VIII) for EC and α-smooth muscle actin for SMC. Arrows indicate Von Willebrand factor (red) and α-smooth muscle actin (green) staining. DAPI (blue) staining refers to fixed cell nuclei (20x magnification). (b) Phase contrast microscopy (10x magnification).
