Skip to main content
. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Virology. 2012 May 8;429(2):155–162. doi: 10.1016/j.virol.2012.04.008

Fig. 4.

Fig. 4

Cooperativity in HIV-1 and Ebola virus particle assembly/release. (A and B). 293T cells were transfected with a fixed amount (25ng) of a Gag-α expression plasmid along with increasing amounts (0ng, 25ng, 50ng and 125ng) of a plasmid expressing untagged Gag. Cells and VLPs were subjected to western blot analysis with anti-Gag and anti-Tubulin antibodies (A) and β-galactosidase assay (B). (C and D) 293T cells were transfected with a fixed amount (50ng) of a plasmid expressing VP40-α along with increasing amounts (0ng 125ng, 250ng and 500ng) of plasmid expressing untagged VP40. Cells and VLPs were subjected to western blot analysis with anti-Myc and anti-Tubulin antibodies (C) and β-galactosidase assay (D).