Figure 2.

Temperature dependence of drug release rates for SPMs in serum and saline.

Notes: A 100 μL aliquot of freshly prepared particles was collected as the zero-hour time point. At subsequent time points, the particles were magnetically retained on a column and the amount of paclitaxel in the nonmagnetic flow-through and in the magnetic particles was measured using an enzyme-linked immunosorbent assay. The amount of drug release is shown as the percentage of drug released compared with the initial amount of drug loaded into the particles, as measured with enzyme-linked immunosorbent assay immediately after encapsulation. SPMs were incubated in saline at 20°C (diamonds), saline at 37°C (squares), serum at 37°C (circles), or serum at 4°C (triangles).

Abbreviation: SPMs, superparamagnetic iron platinum nanoparticles and paclitaxel in a mixture of PEgylated and biotin-functionalized phospholipids.