FIG 3 .

(A) Growth in CCM (chicken cecal medium). 81-176 Str^r, the ΔznuA mutant, and a ΔznuA pznuA mutant were grown in conventional CCM or limited-microbiota CCM statically with and without 20 µM ZnSO₄ for 24 h. The cultures were diluted and plated for C. jejuni recovery, and growth was reported as CFU ⋅ ml⁻¹. Each culture was done in triplicate, and the results shown are representative of three separate trials. (B) Growth in the presence of zinc-binding proteins from conventional chick cecal contents (CC). E1, first elution fraction; BE1, first elution buffer. (C) Growth of 81-176 Str^r, the ΔznuA mutant, and the ΔznuA pznuA mutant after 24 h in MH broth was as described in panel B but with heat treatment as indicated. The cultures were diluted and plated for C. jejuni recovery, and growth is reported as numbers of CFU ⋅ ml⁻¹. Each culture was done in triplicate, and shown are representative results of three separate trials.