TABLE 3 .
Expression ratios of genes in methylotrophic pathways for the acetate-grown ΔmreA strain versus the WWM75 strain of M. acetivorans
| Gene | Transcript abundance (ΔmreA strain/WWM75)a |
|---|---|
| Methyl transfer branch | |
| MA0144–MA0145 (mtmBC-1) | 3.95–3.13 |
| MA0146 (mtbA) | 4.24 |
| MA0455–MA0456 (mtaBC-1) | 0.88–0.49 |
| MA0527, MA0532 (mtbBC-1) | 0.68–1.70 |
| MA0528–MA0529 (mttBC-1) | 0.53–0.57 |
| MA0859 (mtsD) | 1.36 |
| MA0931–MA0932 (mttBC-2) | 6.12–9.27 |
| MA0933–MA0934 (mtbBC-2) | 8.2–10.60 |
| MA1615 (mtaA2) | 0.92 |
| MA1616–MA1617 (mtaBC-3) | 1.21–1.22 |
| MA2424–MA2425 (mtbBC-3) | 0.78–0.95 |
| MA2971–MA2972 (mtmBC-2) | 1.37–1.44 |
| MA4379 (mtaA-1) | 7.83 |
| MA4384 (mtsF) | 1.00 |
| MA4558 (mtsH) | 0.93 |
| Oxidative branch | |
| MA0010 (ftr) | 4.98 (7.07 ± 1.52)b |
| MA0304–MA0309 (fmdEFADCB) | 7.81–10.09 |
| MA0304 (fmdE) | 14.8 ± 4.0b |
| MA0309 (fmdB) | 11.3 ± 2.4b |
| MA1710 (mch) | 1.85 |
| MA3733 (mer) | 6.55 (8.70 ± 2.11)b |
| MA4430 (mtd) | 1.97 |
| Energy conversion | |
| MA1494–MA1507 (fpoPABCDHIJJKLMNO) | 6.35–13.64 |
| MA1495 (fpoA) | 9.34 ± 0.92b |
| MA1498 (fpoD) | 11.9 ± 1.5b |
| MA3732 (fpoF) | 3.91 |
a
Determined by RNA-Seq unless noted otherwise, as indicated in footnote b.
b
Determined by qRT-PCR, calculated using the ΔΔCT method with the 16s rRNA gene used as an invariant control. Values represent the averages and standard deviations of the results of two biological replicate experiments assayed in triplicate. Primer and probe sequences are listed in Table S4 in the supplemental material.