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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: Br J Haematol. 2010 Jun 10;150(5):592–600. doi: 10.1111/j.1365-2141.2010.08268.x

Fig 2.

Fig 2

Representative trajectories of DIDS-biotin labeled band 3 on human erythroblasts. Cells were reacted with DIDS-biotin conjugate, labeled with streptavidin-quantum dots, and imaged by fluorescence video microscopy to monitor the diffusion of individual band 3 molecules. Each sample was imaged at least 100 consecutive frames at a speed of 120 frames/s on (A) fixed mature RBCs, (B) day 8 erythroblasts, (C) day 10 erythroblasts, (D) day 12 erythroblasts, (E) day 15 nucleated erythroblasts, (F) day 15 enucleated erythroblasts, and (G) unfixed mature RBCs at 37° C. The approximate percentages of each cell population that displayed the displayed characteristic trajectory are also indicated.