Table 1.
Microscopic and macroscopic diffusion data of day 8, 10, 12 and 15 erythroblasts and mature RBCs
| RBC type | Dμ (cm2/s) | DM (cm2/s) | Percent immobile† |
Percent constrained‡ |
Percent free |
|---|---|---|---|---|---|
| Fixed (n= 134)¶ |
(6.8 ± 0.1††) ×10−14 (100%)¶¶ |
(5.5 ± 0.2) ×10−14 (100%) |
95‡‡ | 0 | 5 |
| Day 8 erythroblasts (n=296) |
(2.4 ± 0.5) ×10−12 (16%) (6.8 ± 1.0) ×10−11 (84%) |
(1.8 ± 0.1) ×10−12(88%) (4.5 ± 0.7) ×10−11(12%) |
25 | 37 | 38 |
| Day 10 erythroblasts (n=436) |
(2.2 ± 0.4) ×10−12 (14%) (6.3 ± 1.2) ×10−11 (55%) (1.2 ± 0.5) ×10−9 (31%) |
(2.6 ± 0.2) ×10−12 (80%) (3.7 ± 0.5) ×10−10 (20%) |
24 | 34 | 42 |
| Day 12 erythroblasts (n=540) |
(5.5 ± 0.7) ×10−11 (100%) | (2.8 ± 0.1) ×10−12 (92%) (3.1 ± 0.6) ×10−10 (8%) |
18 | 38 | 44 |
| Day 15 erythroblasts nucleated (n=413) |
(2.4 ± 0.8) ×10−12 (20%) (1.2 ± 0.1) ×10−10 (80%) |
(2.9 ± 0.2) ×10−12 (100%) |
30 | 31 | 39 |
| Day 15 erythroblasts Enucleated (n=157) |
(3.6 ± 0.4) ×10−11 (100%) | (1.1 ± 0.1) ×10−12 (100%) |
25 | 36 | 39 |
| Mature RBC (n= 626) |
(1.4 ± 0.4) ×10−11 (77%) (2.2 ± 0.1) ×10−10 (23%) |
(1.7 ± 0.1) ×10−12 (100%) |
24 | 30 | 46 |
The totally immobile fraction is defined as the fraction of band 3 molecules that were found to have a DM value less than or equal to the slowest 95% of DM values measured for band 3 on fixed erythrocytes (DMImm); i.e. the fraction of band 3 that diffuses similar to band 3 on fixed cells.
Percent constrained includes all trajectories that were classified as undergoing confined or hop diffusion and for which DM > DMImm.
Total number of trajectories analysed.
Errors are given as the standard error of the mean.
Defined as such.
Reflects the fraction of total area contained in each peak of the Gaussian fit to the distribution. For those distributions that fit best with a single peak it is understood that 100% of the area is contained within that peak