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. 1978 Jan;5(1):13–21. doi: 10.1093/nar/5.1.13

Preparative separation of the complementary strands of DNA restriction fragments by alkaline RPC-5 chromatography.

H Eshaghpour, D M Crothers
PMCID: PMC341958  PMID: 25414

Abstract

High pressure reversed phase chromatography (RPC-5) at pH 12 was used for preparative separation of the complementary strands of the smaller DNA fragments which are generated by the Hae III restriction endonuclease digestion of Col El DNA. A single high pressure RPC-5 chromatographic step at neutral pH served to purify duplex fragments 70, 172, 250 and 440 base pairs long; each of these yielded two elution peaks upon chromatography under alkaline denaturing conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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