Table 2.
Summary of H/2H exchange data for PKA catalytic subunit.
| Deuteration (2min.) |
|||||||
|---|---|---|---|---|---|---|---|
| Fragment of PKA C-subunit (m/z) |
Number of amides |
C-subunit (apo) |
C-subunit (+Mg2+ATP) |
RIIβ(108–402):C (apo) |
RIIβ(108–402):C (+Mn2+AMP-PNP) |
RIIβ(108–268):C (+Mn2+AMP-PNP) |
|
| C1 | 27-40 (1643.88) | 12 | 7.8 ± 0.0 | 10.4 ± ND | - | 9.8 ± 0.1 | 11.7 ± 0.3 |
| C2 | 44-54 (1194.65) | 10 | 6.1 ± 0.8 | 4.8 ± 0.4 | 3.8 ± 0.0 | 3.8 ± 0.2 | 5.3 ± 0.2 |
| C3 | 41-54 (1584.80) | 13 | 7.6 ± 0.0 | 7.2 ± 0.1 | 6.8 ± 0.0 | 5.3 ± 0.1 | 7.7 ± 0.2 |
| C4 | 66-83(2113.23) | 17 | 6.8 ± 0.3 | 5.0 ± ND | 2.1 ± 0.0 | 5.3 ± 0.1 | 7.7 ± 0.2 |
| C5 | 92-100 (1088.66) | 8 | 2.3 ± 0.2 | 1.7 ± 0.2 | 1.1 ± ND | 2.2 ± 0.0 | 2.0 ± 0.2 |
| C6 | 133-145(1628.89) | 11 | 3.7 ± 0.1 | 2.6 ± 0.4 | 1.0 ± 0.1 | 1.4 ± 0.2 | 2.3 ± 0.0 |
| C7 | 164-172(1147.61) | 7 | 1.9 ± 0.0 | 0.6 ± 0.0 | - | - | - |
| C8 | 163-172(1260.70) | 8 | 2.2 ± 0.3 | 1.0 ± 0.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.3 ± 0.0 |
| C9 | 164-174(1373.78) | 9 | 1.9 ± 0.0 | 2.0 ± 0.3 | 1.1 ± 0.1 | 0.4 ± 0.1 | 0.9 ± 0.1 |
| C10 | 212-221(1167.58) | 9 | 4.9 ± 0.8 | 3.4 ± 0.2 | 3.1 ± 0.4 | 2.4 ± 0.0 | 3.4 ± 0.2 |
| C11 | 247-261(1793.97) | 13 | 7.3 ± 0.6 | 6.4 ± 0.6 | 3.7 ± 0.1 | 4.0 ± 0.0 | 6.2 ± 0.3 |
| C12 | 278-289(1347.75) | 11 | 6.1 ± ND | 4.4 ± 0.2 | 1.2 ± ND | 1.5 ± 0.1 | 6.1 ± 0.3 |
| C13 | 303-326(2676.45) | 20 | 12.0 ± 0.3 | 11.1 ± 0.2 | 8.4 ± 0.3 | 7.5 ± ND | 11.2 ± 0.3 |
| C14 | 303-327(2823.52) | 21 | - | 12.4 ± 0.2 | - | - | 12.6 ± 0.3 |
| Deuteration (2min.) |
|||||||
| Sequence of RIIβ(m/z) | Number of amides | RIIβ(108–402)(apo) | RIIβ(108–268): (apo) | RIIβ(108–268):C(+Mn2+AMP-PNP) | RIIβ(108–402):C (apo) | RIIβ(108–402):C(+Mn2+AMP-PNP) | |
| R1 | 226-24 (1704.97) | 14 | 0.9 ± ND | 2.5 ± 0.1 | 3.6 ± 0.0 | 0.7 ± 0.2 | 1.4 ± 0.0 |
| R2 | 253-26 (2281.31) | 17 | 10.7 ± ND | 11.8 ± 0.1 | 10.8 ± 0.5 | 0.5 ± 0.2 | 0.6 ± 0.0 |
| R3 | 319-33 (1790.07) | 14 | 12.8 ± 0.1 | 11.2 ± 0.1 | |||
| R4 | 339-34 (1079.58) | 8 | 1.2 ± 0.1 | 2.5 ± 0.2 | 2.0 ± 0.1 | ||
| R5 | 339-35 (1525.79) | 12 | 3.1 ± 0.0 | 4.0 ± 0.1 | 4.0 ± 0.1 | ||
| R6 | 354-37 (1824.06) | 15 | 3.5 ± ND | 4.5 ± 0.1 | 5.1 ± 0.1 | ||
| R7 | 380-388 (1047.6) | 7 | 2.7 ± ND | 2.4 ± 0.0 | 2.3 ± 0.1 | ||
*
ND: Not determined
Averages and standard deviations were calculated with measurements from three independent experiments for most peptides. Due to high noise in data sets for certain peptides, fewer measurements were obtained and consequently no standard deviations were calculable.