Figure 1. Diva acts through the intrinsic mitochondrial death pathway. a.

PC12 cells were harvested for total RNA isolation and quantitative real-time PCR to determine the changes in mRNA expression levels of Diva, Bcl2 and Bax following 100 ng/ml of FasL treatment. RNA was extracted 0 hr, 8 hrs, 24 hrs and 48 hrs after treatment and the fold changes were calculated with GAPDH as the internal control. b Fold changes of Diva and Bcl2 after addition of 30 nM of H₂O₂ for 0 hrs, 2 hrs, 4 hrs and 8 hrs. c Fold change of Bax after 30 nM of H₂O₂ treatment across 8 hrs. Bar graphs represent mean ± SD from three independent experiments. *p<0.05, **p<0.01. d PC12 cells were harvested to determine the protein expression levels of Diva, Cleaved Caspase 8, Bax, Bcl2 and Cleaved Caspase 3. Western blot was conducted on the total protein extracted following 100 ng/ml of FasL treatment for 0 hrs, 8 hrs, 24 hrs and 48 hrs. e PC12 cells were harvested to determine the protein expression levels of Diva, Cleaved Caspase 9, Bax, Bcl2 and Cleaved Caspase 3. Western blot was conducted on the total protein extracted following 30 nM of H₂O₂ treatment for 0 hrs, 2 hrs, 4 hrs and 8 hrs. B-actin was used as the loading control.