Skip to main content
. 2012 Aug 15;7(8):e43180. doi: 10.1371/journal.pone.0043180

Figure 3. Knock down of Diva increases mitochondrial pathway protein expression and mitochondrial membrane disruption.

Figure 3

a–b PC12 cells were harvested to determine the protein expression levels of Diva, cleaved caspase 9, cleaved caspase 3 and cytochrome C after Diva silencing. Western blot was conducted on the total protein extracted three days after Diva knock down. DF, Neg, s1 and s2 represent transfection reagent only, negative control siRNA, Diva siRNA1 and Diva siRNA2 respectively. c Western Blot showing protein levels of Diva, cleaved caspase 3 and cleaved caspase 9 three days after Diva silencing in BMSCs. B-actin was used as the loading control. d Mitochondrial membrane potential of PC12 was assessed by staining transfected cells with the JC-1 assay stain. The top panel shows PC12 which had been transfected with the negative control siRNA, while the bottom panel shows PC12 48 hrs after Diva silencing. Green stained for apoptotic cells with low mitochondrial potential while red stained for live cells with high mitochondrial potential. e JC-1 staining of BMSCs 48 hrs after transfection with negative control siRNA and Diva siRNA. The top panel shows BMSCs which had been transfected with the negative control siRNA, while the bottom panel shows BMSCs 48 hrs after Diva silencing. Bar  = 100 µm.