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. 2012 Aug 15;7(8):e43180. doi: 10.1371/journal.pone.0043180

Figure 4. Diva silencing increases the proportion of apoptotic cells.

Figure 4

a Flow cytometry performed on live PC12 cells within 1 hr of Annexin V staining. PC12 cells were stained 48 hrs after transfection with negative control and Diva siRNA. b Flow cytometry performed on live PC12 cells within 1 hr of PI staining. PC12 cells were stained 48 hrs after transfection with negative control and Diva siRNA. c Flow cytometry result of Annexin V stained negative control siRNA and Diva siRNA transfected BMSCs. d Flow cytometry result of PI stained negative control siRNA and Diva siRNA transfected BMSCs. Annexin V and PI staining were performed on live BMSCs 48 hrs after transfection. e–f Graphs showing the relative increase in Diva siRNA transfected PC12 cells that had taken up the Annexin V and PI stains respectively. g–h Graphs showing the relative increase in Diva siRNA transfected BMSCs that had taken up the Annexin V and PI stains respectively. Results were statistically significant with p-values <0.01.