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. 2012 Aug 15;7(8):e43175. doi: 10.1371/journal.pone.0043175

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© 2012 Hatzios et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Luminescence generated by wild-type (),Δche2 (), and Δche1che2che3 () V. cholerae co-expressing CheY3-RlucN and CheZ-RlucC. Data from three biological replicates were normalized by optical density, averaged, and reported as a percentage of the highest initial signal intensity. (B) Western blot analysis of CheY3-RlucN/CheZ-RlucC expression in samples from (A). Only the CheZ-RlucC fusion protein was detected by the monoclonal anti-Rluc antibody.

Inline graphic — (A) Luminescence generated by wild-type (),Δche2 (), and Δche1che2che3 () V. cholerae co-expressing CheY3-RlucN and CheZ-RlucC. Data from three biological replicates were normalized by optical density, averaged, and reported as a percentage of the highest initial signal intensity. (B) Western blot analysis of CheY3-RlucN/CheZ-RlucC expression in samples from (A). Only the CheZ-RlucC fusion protein was detected by the monoclonal anti-Rluc antibody.