Figure 1.
Expression of Toll pathway marker genes after exposure ofAedes aegyptiAag-2 cells to Gram-positive (M. luteus) or negative (E. cloacae) bacteria or to Zymosan. Cells were incubated with the indicated amounts of the indicated stimuli for 24 hours and the expression of REL 1(A), MyD88 (B), cactus (C) or serpin (D) was analyzed by qPCR using RP-49 gene as endogenous control. Each bar represents the relative expression and standard error of the analyzed genes, calculated as described in Methods section.