Table 1.
Primers used in this study
| Accession number | Gene | Primer sequence | Melting temperature (°C) | R2efficiency Curve | Primer efficiency |
|---|---|---|---|---|---|
| AAEL007696 |
aaREL 1 For |
GACTCGTCGGAGCTGAAATC |
81.1 |
0.9989 |
1.1516 |
| aaREL 1 Rev |
CGGTTTGTTCAGGTTGTTGA |
||||
| AAEL007624 |
aaREL 2 For |
TCTGTCGGCAGATGAAGTGA |
79.7 |
0.9999 |
1.1144 |
| aaREL 2 Rev |
GCACTGGAATGGAGAATCAAA |
||||
| AAEL000709 |
Cactus For |
TCTTGCGTTGAAGTGAGTGG |
79.2 |
0.993 |
1.034 |
| Cactus Rev |
GACCCTCTGAAAGGGAAAGG |
||||
| AAEL003841 |
Defensin For |
GATTCGGCGTTGGTGATAGT |
81.9 |
0.9992 |
0.9955 |
| Defensin Rev |
TTATTCAATTCCGGCAGACG |
||||
| AAEL010083 |
IMD For |
TCGTCAAACTCGGTTTTCCT |
78.9 |
0.9925 |
1.1068 |
| IMD Rev |
TGGCGGAGTTGAAGGTAAAG |
||||
| AAEL007768 |
Myd88 For |
CGATGCGTTCATTTTGTTTG |
76.8 |
0.9889 |
1.03 |
| Myd88 Rev |
CACCGCTCAGAAATCAGCTT |
||||
|
AAT45939 |
RP49 For |
GCTATGACAAGCTTGCCCCCA |
83.7 |
0.9879 |
1.2002 |
| RP49 Rev |
TCATCAGCACCTCCAGCT |
||||
| AAEL007765 |
Serpin For |
ACGTGATGGATTGGATGGAG |
79.2 |
0.9995 |
1.0149 |
| Serpin Rev |
GTGCCTGCACTTGTTTCTGA |
||||
| AAEL009692 |
STAT For |
CACACAAAAAGGACGAAGCA |
75.7 |
1 |
1.1797 |
| STAT Rev |
TCCAGTTCCCCTAAAGCTCA |
||||
| AAEL001794 |
TEP For |
ATTTTTGACGGCTTTTGTGG |
78.9 |
0.9992 |
1,0604 |
| TEP Rev | TGGATTACTTGCCCCACTTC |