Figure 4. Complementation of eap deletion.

S. aureus strain Newman wild-type (WT), mAH12 (eap), mAH12pll39 (pll39) and mAH12plleap (plleap) were assessed for Eap production by SDS-PAGE (A) and Western blotting using anti-Eap antibodies (B). The position of bands corresponding to Eap is indicated (<). The growth rates of the four strains were compared to ensure that complementation did not have an adverse effect (C).