Table 1. Strains used in this study.

Strain	Relevant characteristics	Reference
E. coli
DH5α	Host strain for pll39 undergoing genetic manipulation.
Pll39	Plasmid allowing for single copy integration of DNA onto the S. aureus chromosome.	[34]
plleap	Pll39 containing the eap gene and flanking DNA including promoter region.	This study
S. aureus
Phage Φ11	Used for transduction of DNA between S. aureus strains.	[48]
pFnBA4	Plasmid containing the entire fnbA gene and promoter.	[49]
RN4220	Restriction deficient sub-cloning host strain. Tolerates exogenous DNA.	[50]
Newman WT	Wild type strain. Expresses Eap.	[51]
Newman mAH12	Newman Δeap mutant strain (eap::ermB).	[16]
Newman mAH12(pll39)	Δeap geh::pll39. eap deficient mutant strain with empty pll39 plasmid inserted into the L54a attBinsertion site within the geh gene.	This study
Newman mAH12(plleap)	Δeap geh::plleap. eap deficient mutant strain with pll39 plasmid containing the eap gene inserted into theL54a attB insertion site within the geh gene. Expresses Eap.	This study
Newman WT pFnBA4	Newman wild-type strain expressing FnBPA.	This study
DU5873	Newman Δspa mutant strain (eap::Tcr).	[52]
DU5852	Newman ΔclfA mutant strain (clfA::Tn917 Emr).	[53]
DU5855	Newman Δcoa mutant strain (coa::Tcr).	[54]
DU5917	Newman Δcps mutant strain (eap::Tn917 Emr).	[55]