Table 1. Sequenced Alpha-Proteobacterial strains containing GTA gene cassettes and screened for GTA production over time.
Isolate Name | ATCC strain number | Maximum GTA production | Genome Accession Number (Refseq) | Locus tag- GTA Terminase/GTA ORF 15 | Growth Media | Preferred temp |
Rhodobacter capsulatus SB1003WT | Gift from Oregon State University | yes (type strain, 105 gene transfer units ml−1), Solioz et.al., 1975 | NC_ 014034 | RCAP_rcc01683 RCAP_rcc01698 | PYE, YPS, LB or OM | 30°C aerobic, or anaerobic with light |
Roseobacter denitrificans OCH114 | ATCC® 33942TM | not observed | NC_ 008209 | RD1_3034 RD1_3016 | Marine Broth 2216 | 26°C aerobic |
Oceanicola granulosus HTCC2516 | ATCC® BAA861TM | yes, (2.9×106 ml−1) | NZ_ AAOT00000000 | OG2516_04279 OG2516_04219 | Marine Broth 2216 | 30°C aerobic |
Roseovarius nubinhibens ISM | ATCC® BAA591TM | yes, (1×109 ml−1) | NZ_ AALY00000000 | ISM_05135 ISM_05210 | Marine Broth 2216 | 30°C aerobic |
Ruegeria (Silicibacter) pomeroyi DSS-3 | ATCC® 700808TM | yes, (×108 ml−1) Biers et.al., 2008 | NC_ 003911 | SPO2266 SPO2250 | Marine Broth YTSS | 28°C aerobic |
Oceanicaulis alexandrii | Gift from Oregon State University | not observed | NZ_ AAMQ00000000 | OA2633_14805 OA2633_14855 | Marine Broth 2216 | 20°C aerobic |
Aurantimonas manganoxydans SI85-9A-1 | ATCC® BAA-1229 | not observed | NZ_ AAPJ00000000 | SI859A1_01033 SI859A1_08129 | #2584 Broth: buffered marine media with Mn | 30°C aerobic |
Parvularcula bermudensis | ATCC® BAA594TM | pending | NC_ 014414 | PB2503_08059 PB2503_08129 | Marine Broth 2216 | 30°C aerobic |
Nitratireductor , strain 44B9s | Isolated from Symbiodinium associated with a Gorgonian | yes (2×109 ml−1) | N/A | N/A | Marine Broth 2216 | 26°C aerobic |
Ruegeria mobilis, strain 45A6 | Isolated from a Clade D2 Symbiodinium associated with a Foraminifera | yes (1×109 ml−1) | Pending | Pending | Marine Broth 2216 | 26°C aerobic |
The strains screened for this study are indicated in bold type. Maximum abundance of GTA particles observed in parentheses.