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PMA and vehicle effect on the migration of HaCaT (A) and SCC-9 (B) cells after 7, 24 and 48 h. Cells grown in 12-well plates to confluence were scraped with p200 pipette tip. The closure was measure after 0, 7, 24 and 48 h and normalized to the control (vehicle: DMSO). Two independent experiments were performed with triplicates. Columns represent mean ± SD (n = 2) and * indicates p<0.01, compared to control (vehicle).
