Figure 5. Gelatinase activity was increased with PMA stimuli.

Representative 1D-zymography of conditioned media (12 µg of proteins) of HaCaT and SCC-9 cells treated or not with 50 ng/ml PMA two times with 12 h interval, collected after 24 h. The gelatinase activity of immunoprecipitated MMP-2 was used as positive control. Numbers on the left indicate the molecular mass marker mobility (A). The densitometry of clear areas showed the increase of activity after PMA treatment (B). Columns represent mean ± SD (n = 3) and * indicates p<0.05.