Table 1. Identification of proteins in the conditioned media by LC-MS/MS according to the ratio of quantitative value.
| Accession Number | Protein Identification | HaCat + PMA/HaCaT | SCC-9+ PMA/SCC-9 | SCC-9/HaCaT | SCC-9+PMA/ HaCaT+PMA |
| Extracellular Matrix Proteins | |||||
| IPI00374563 | Agrin | 7.88 | absent | only in HaCaT | only in HaCat+PMA |
| IPI00022418 | Isoform 1 of Fibronectin | absent | only in SCC+PMA | absent | only in SCC+PMA |
| IPI00003951 | Isoform 1 of Laminin subunit alpha-3 | 5.09 | 14.17 | 0.46 | 1.29 |
| IPI00015117 | Isoform Long of Laminin subunit gamma-2 | only in HaCaT + PMA | only in SCC+PMA | absent | 0.70 |
| IPI00783665 | Laminin subunit alpha-5 | only in HaCaT + PMA | absent | absent | only in HaCat+PMA |
| IPI00299404 | Laminin subunit beta-3 | 2.36 | Only in SCC+PMA | only in HaCaT | 0.39 |
| Secreted Proteins | |||||
| IPI00011229 | Cathepsin D | 1.28 | 1.30 | 0.67 | 0.68 |
| IPI00297487 | Cathepsin H | 1.54 | absent | only in HaCaT | only in HaCat+PMA |
| IPI00021738 | Collagenase 3 (MMP-13) | only in HaCaT + PMA | absent | absent | only in HaCat+PMA |
| IPI00023673 | Galectin-3-binding protein | absent | only in SCC | only in SCC | absent |
| IPI00023728 | Gamma-glutamyl hydrolase | only in HaCaT | absent | only in HaCaT | absent |
| IPI00008561 | Interstitial collagenase | only in HaCaT + PMA | absent | absent | only in HaCat+PMA |
| IPI00026314 | Isoform 1 of Gelsolin | only in HaCaT | absent | only in HaCaT | absent |
| IPI00291262 | Isoform 1 of Clusterin | only in HaCaT | absent | only in HaCaT | absent |
| IPI00029658 | Isoform 1 of EGF-containing fibulin-like extracellular matrix protein 1 | only in HaCaT + PMA | absent | absent | only in HaCat+PMA |
| IPI00387168 | Isoform 1 of Proprotein convertase subtilisin/kexin type 9 | 1.57 | absent | only in HaCaT | only in HaCat+PMA |
| IPI00783625 | Isoform 1 of Serpin B5 | 3.03 | absent | only in HaCaT | only in HaCat+PMA |
| IPI00013890 | Isoform 1 of 14-3-3 protein sigma | 0.59 | absent | only in HaCaT | only in HaCat+PMA |
| IPI00480121 | Kallikrein-10 | only in HaCaT + PMA | only in SCC+PMA | absent | 10.43 |
| IPI00293276 | Macrophage migration inhibitory factor | 0.16 | 1.12 | 0.86 | 5.97 |
| IPI00216691 | Profilin-1 | absent | 0.84 | only in SCC | only in SCC+PMA |
| IPI00013895 | Protein S100-A11 | only in HaCaT | absent | only in HaCaT | absent |
| IPI00294879 | Ran GTPase-activating protein 1 | only in HaCaT | absent | only in HaCaT | absent |
| IPI00009342 | Ras GTPase-activating-like protein IQGAP1 | 0.34 | absent | only in HaCaT | only in HaCat+PMA |
| IPI00296099 | Thrombospondin-1 | 3.09 | only in SCC+PMA | only in HaCaT | 0.20 |
| IPI00018219 | Transforming growth factor-beta-induced protein ig-h3 | 0.95 | 1.00 | 0.39 | 0.41 |
| Membrane-bound proteins | |||||
| IPI00218918 | Annexin A1 | 0.96 | 2.19 | 0.85 | 1.93 |
| IPI00024095 | Annexin A3 | absent | only in SCC | only in SCC | absent |
| IPI00329801 | Annexin A5 | absent | 8.06 | only in SCC | only in SCC+PMA |
| IPI00414320 | cDNA FLJ55482. highly similar to Annexin A11 | Only in HaCaT | absent | only in HaCaT | absent |
| IPI00015688 | Glypican-1 | 2.07 | absent | only in HaCaT | only in HaCat+PMA |
| IPI00010271 | Isoform A of Ras-related C3 botulinum toxin substrate 1 | absent | only in SCC | only in SCC | absent |
| IPI00218474 | Isoform 1 of Beta-enolase | 4.12 | 0.07 | 74.08 | 1.25 |
| IPI00465248 | Isoform alpha-enolase of Alpha-enolase | 1.12 | 0.28 | 7.44 | 1.86 |
| IPI00031030 | Isoform 1 of Amyloid-like protein 2 | only in HaCaT + PMA | absent | absent | only in HaCat+PMA |
| IPI00418169 | Isoform 2 of Annexin A2 | 1.04 | 0.85 | 0.92 | 0.75 |
| IPI00871158 | Isoform 2 of Annexin A8-like protein 2 | absent | only in SCC | only in SCC | absent |
| IPI00297160 | Isoform 12 of CD44 antigen | 3.18 | only in SCC+PMA | only in HaCaT | 1.31 |
| IPI00027493 | Isoform 2 of 4F2 cell-surface antigen heavy chain | 0.23 | 3.46 | 1.58 | 24.32 |
| IPI00554711 | Junction plakoglobin | 1.53 | absent | only in HaCaT | only in HaCat+PMA |
| IPI00026952 | Plakophilin-3 | 3.05 | only in SCC | 1.18 | only in HaCat+PMA |
| IPI00002441 | Syndecan-1 | absent | only in SCC+PMA | absent | only in SCC+PMA |
| IPI00011564 | Syndecan-4 | 1.01 | only in SCC+PMA | only in HaCaT | 8.18 |
| Intracellular proteins | |||||
| IPI00020599 | Calreticulin | absent | only in SCC | only in SCC | absent |
| IPI00298237 | cDNA FLJ56402. highly similar to Tripeptidyl-peptidase 1 | 1.81 | absent | only in HaCaT | only in HaCat+PMA |
| IPI00843975 | Ezrin | 0.64 | 2.27 | 0.34 | 1.19 |
| IPI00163187 | Fascin | 1.22 | 2.21 | 0.39 | 0.71 |
| IPI00024067 | Isoform 1 of Clathrin heavy chain 1 | absent | 2.53 | only in SCC | only in SCC+PMA |
| IPI00014898 | Isoform 1 of Plectin-1 | absent | only in SCC | only in SCC | absent |
| IPI00291175 | Isoform 1 of Vinculin | only in HaCaT + PMA | absent | absent | only in HaCat+PMA |
| IPI00219365 | Moesin | only in HaCaT + PMA | only in SCC+PMA | absent | 1.01 |
| IPI00915869 | Putative uncharacterized protein MDH1 | absent | only in SCC | only in SCC | absent |
| IPI00298994 | Talin-1 | Only in HaCaT | 1.53 | 24.56 | only in SCC+PMA |
The HaCaT and SCC-9 cells were treated with PMA for 24 h, the media were collected, the proteins were digested with trypsin and analyzed by LC-MS/MS. The data were submitted to Mascot search engine and the .dat files from Mascot output were analyzed in Scaffold Q+, which calculates the quantitative value by normalizing spectral counts across the experiments. The ratio of quantitative value of the PMA-stimulated cells/DMSO-treated cells for each protein is shown in the table. The proteins exclusively found in one condition are indicated as “only” and the proteins that are not present are indicated as “absent”.